Phosphorus-31 n.m.r. spectroscopy

Comparison of the whole lipopolysaccharide antigens of the seven immunotypes by phosphorus-31 n.m.r. spectroscopy ( 5) does provide a tool for differentiating the seven types, as shown in Figure 1, where it may be seen that each individual immunotype gives a characteristic J1P-n.m.r. profile that could be used as a diagnostic reference for the lipopolysaccharide of that individual immunotype. The 31p-n.m.r. spectra have also been used to glean information as to the chemical nature of the phosphate groups in the lipopolysaccharides (5,14—16). 

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