Phosphorus methods in nucleotide chemistry

NMR spectroscopy is perhaps not quite so popular as F in pharmaceutical development because of the lower number of drug substances containing phosphorus. However, it has importance in biochemistry and the same potential for impurity quantitation as F, and there are many structural problems where it can help, for example in Wittig reactions, ylides, phosphate esters, nucleotides and phospholipids [57]. Two-dimensional methods for phosphorus have been reviewed [58]. The latter reference also reviews H-X, X-X and X-Y correlation experiments for other heteronuclei. Given the relative dearth of tertiary literature on phosphorus NMR in chemistry, the book by Berger etal. [38] is particularly useful. 

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