Phosphoribosylanthranilate isomerase PRAI

Phosphoribosylanthranilate isomerase (PRAI) (EC 2.4.2.18) catalyzes the penultimate step in tryptophan biosynthesis. In contrast to the enzyme firom meso-philes, TmPRAI is again a homodimer showing thermal stability up to 95°. Under... 

In the biosynthesis pathways of histidine and tryptophan, the enzymes HisA (N -[(5 -phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide isomerase) and TrpF (PRAI,phosphoribosylanthranilate isomerase) (Figure 16.13), both of which are ()3a)8-barrels, both catalyze Amadori rearrangements of a ther-molabile aminoaldose into the corresponding aminoketose (Figure 16.13). 

Abbreviations ADCA, adipyl-cephalosporic acid epPCR, error-prone PCR IGPS, indole-3-glycerol phosphate synthase MO, monooxygenase MS, mutator strain PRAI, phosphoribosylanthranilate iso-merase ProFARI, N -[5-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxylamide ribonucleotide isomerase SDM, site-directed mutagenesis, StEP, staggered extension process... 

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