Phosphopyruvate hydratase

Sodium fluoride (104) (1-10 mM) inhibits two enzymes of glycolysis the enolase (phosphopyruvate hydratase) and pyruvate kinase. Therefore, aerobic glucose utilization and lactate formation are blocked. 

Scheme 11.25. A representation of the conversion of 2-phosphoglycerate to phosphoenol-pyruvate with catalysis by phosphopyruvate hydratase (EC 4.2.1.11).

Then, in the presence of the enzyme enolase (phosphopyruvate hydratase, EC 4.2.1.11) and as shown in Scheme 11.25, dehydration of the 2-phospho-D-glycerate (2-phosphoglycerate) occurs to produce the phosphate enol of pyruvic acid, phos-phoenolpyruvate (PEP). 

The Citric Acid Cycle (or the Tricarboxylic Acid [TCA] Cycle or the Krebsi Cycle). It will be recalled (Schemes 11.24-11.26) that the enzyme phosphoglycerate mutase (EC 5.4.2.1) acts on PGA (obtained from, e.g., fructose-1,6-bisphosphate) to produce the isomeric, 2-phosphoglycerate and that phosphopyruvate hydratase (EC 4.2.1.11) then converts the 2-phosphoglycerate to phosphoenolpyruvate. 

Fig. 15.2 Pyruvate metabolism, gluconeogenesis and glycolysis. Key enzymes shown are 1, fructose 1-phosphate aldolase (EC 4.1.2.13) 2, fructose 1,6-bisphosphate aldolase (EC 4.1.2.13) 3, NAD rglyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12) 4, 3-phosphoglycerate kinase (EC 2.T.2.3) 5, phosphoglyceromutase (EC 2.7.5.3) 6, phosphopyruvate hydratase (enolase) (EC 4.2.1.11) 7, pyruvate kinase (EC 2.7.1.40) 8, pyruvate carboxylase (EC 6.4.1.1) 9, phosphopyruvate carboxylase...

Aconitate hydratase [Fe4S4]— aconitase Phosphopyruvate hyd ratase— enolase ... 

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