Phenylacetone monooxygenase

Figure 3 Illustration of overlapping substrate specificities of four BVMOs. Cyclohexanone monooxygenase (CHMO), cyclopentanone monooxygenase (CPMO), 4-hydroxyacetophenone monooxygenase (HAPMO) and phenylacetone monooxygenase (PAMO). For each enzyme, several typical substrates are...

Genomes were surveyed for the presence of putative type I BVMOs by (1) searching for sequence homologs of phenylacetone monooxygenase and (2) filtering for sequences that contain the type I BVMO-sequence motif2 ... 

Structural and kinetic studies on phenylacetone monooxygenase have revealed the importance of a conserved arginine in the reactivity with organic substrates (37). 

Torres DE Pazmino, Baas BJ, Janssen DB, Fraaije MW. Kinetic mechanism of phenylacetone monooxygenase from Thermobifida fusca. Biochemistry 2008 47 4082-4093. 

The mechanisms of a few Baeyer-Villiger monooxygenases have been studied in detail. The catalytic cycles of cyclohexanone monooxygenase from Acinetobacter NCIB 9871 and phenylacetone monooxygenase from Thermobifida fusca are similar to each other and to that of As with FMO, wasteful and toxic... 

As the flavin-dependent enzyme, a mutant of phenylacetone monooxygenase, PAMO-P3, was chosen [104], which had previously been engineered to catalyze the enantioselective BV reaction of substituted ketones. The unnatural regeneration conditions did not impair the inherent stereoselectivity of PAMO-P3, suggesting an unaltered mechanism of the actual oxidation reaction. The present light-driven BV reaction is expected to work just as well using other flavin-dependent BVMOs such as CHMO, which accepts a broader range of substrates. 

---