Phenyl thiocarbamyl

Several techniques have been developed to analyse amino acids. The following procedure describes one of the most frequently used methods for the determination of amino acid concentration, the PITC method. Free amino acids in medium samples are derivatized with phenylisothiocyanate (PITC) to produce phenyl-thiocarbamyl (PTC) amino acids. After separation by reverse-phase HPLC, the PTC amino acids are detected using a UV spectrophotometer at 254 nm. 

Molnar-Perl 1 (1994) Advances in the high-performance liquid chromatographic determination of phenyl-thiocarbamyl amino acids. Journal of Chromatography A 661 43-50. 

Kazmierczak, D. Ciesielski, W. Zakrzewski, R. Separation of amino acids as phenyl thiocarbamyl derivatives by normal- and reversed-phase thin-layer chromatography. J. Planar Chromatogr. -Mod. TLC 2005,18, 427-431. 

Quinazoline-2-thione-4-one (522) and its 3-phenyl derivative coordinate to Ni11 after deprotonation via the S and the NI donor resulting in a square planar complex.1372 The related 2-isopropylquinoline-8-thiolate (523) forms a distorted tetrahedral complex.1 73 For deprotonated 2-thiocarbamyl-thiazoline (HL = (524)) the octahedral complex [NiL(H20)4]+ is favored over the square planar [NiL2] complex.1374... 

In retrospect, the development and standardization of S-9 has centered around a limited number of chemical types, i.e., iV-nitrosamines, aromatic amines, and polycyclics. Accordingly, the ability of microbial systems supplemented with S-9 to detect these chemicals has been rather consistent. However, the converse also appears true chemicals, other than those types with which the testing has been standardized, that are metabolized in vivo to their active forms are not as consistently detected in such in vitro testing. While further discussion of this point is reserved for later, suffice it here to note that, in general, poorly activated types include azonaphthol dyes carbamyl and thiocarbamyl compounds phenyls polyhalogenated aromatics, cyclics, and aliphatics benzodioxoles and symmetrical hydrazines. 

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