PHB Production by Mixed Culture

There are a few studies on PHA production by mixed cultures using propionate, butyrate, lactate, succinate, pyruvate, malate, ethanol, glutamate and aspartate and glucose, but no studies on the optimization of nutrient removal and PHA production by mixed cultures are available. 

During the EBPR anaerobic phase, carbon substrates such as acetic and propionic acids are taken up to biosynthesize PHV. The energy and reduction equivalent required for PHA biosynthesis are provided by the degradation of intracellular saved polyphosphate (poly-P) and glycogen as well as substrate degradation in the tricarboxylic add cycle. The bulk phosphorus concentration in the anaerobic period is, therefore, increased with time. In the 

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