Phase Synthesis of PEG-Conjugated Oligonucleotides

Many advantages arise from this S5mthetic process. First of all, the oligonucleotide synthesis is carried out in homogeneous media since the chain to be synthesised is linked to a soluble supporting polymer. The polymer-bound product is recovered from the reaction mixture by a simple precipitation that allows rapid and easy elimination of excess reagents and 

The same phosphoramidite-based chemistry, widely employed in the 

To verify the effect of the different shape of the PEG unit, the same oligonucleotide has been conjugated to a new branched polyethylene glycol. The synthetic data are quite similar to those of the linear MPEG-conjugate, save some reduced yield during the first synthetic steps, likely due to some steric hindrance of the reactive functionality of the branched polymer. 

A first biological characterisation of these high-molecular mass MPEG-conjugated oligonucleotides has been performed by their thermal 

MPEG diPhn MPEG- MPEG-8mer 12mer 12itier + diPhn 8mer 

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