Phase refinement solvent flattening

Tel. 44-925-603528, fax 44-925-603100, e-mail ccp4 daresbury.ac.uk Suite of almost 100 protein crystallography programs for data processing, scaling, Patterson search and refinement, isomorphous and molecular replacement, structure refinement, such as PROLSQ, phase improvement (solvent flattening and symmetry averaging), and presentation of results, such as SURFACE for accessible surface area. Available via ftp from anonymous ... 

The method of Karle (1980) was used for phasing (section 9.4) as implemented by Hendrickson (1985). Prior to fitting a molecular model, the noise in the electron density map was reduced by solvent flattening (figure 9.16). The interpreted map followed by refinement of the model yielded an -factor of 22% at 1.8 A resolution. 

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