Phage binding

If quantitative analysis of antibody-phage binding is desirable, it is possible to perform an ELISA procedure using purified phage as antigen. 

Fig. 3 Left Schematic representation of a DNA-loaded triblock-based polymersome. The virus, a X phage, binds a LamB protein and the DNA is transferred across the block copolymer membrane. Right An electron micrograph of negatively stained complexes formed between X phage and vesicles bearing LamB proteins at 37° C. The X phage (large structure on the top left comer) is attached to one vesicle via its tail. Ref. [27]. Copyright (2002) National Academy of Sciences, U.S.A...

The following calculations are used to compare phage binding to cells ... 

Prophage PA2 Replacement of the PA-2 binding protein (OmpC) with a non-phage binding equivalent porin to prevent superinfection... 

Schluesener, H. J. and Tan, X. 2004. Selection of recombinant phages binding to pathological endothelial and tumor cells of rat glioblastoma by in-vivo display. J. Neurol. Sci. 224 77-82. 

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