PH responsive polymer-based biosensors

Biosensors are often based on the use of an enzyme with specific response. Polymerization of pyrrole can be carried out in aqueous solution at moderate pH values. In those conditions enzymes can be entrapped in the growing film of the polymer formed on the electrode. This has been applied in particular to the glucose-oxidase enzyme, in order to build a glucose sensor [412 18]. 

The research program included also both the theory and practical aspects of ion-selective electrodes. Important part of these studies was the research oti the selectivity of such sensors and interferents and the change of selectivity imder influence of various factors. Potentiometric enzymatic sensors and sensors based oti pH-electrodes were developed and used in clinical chemistry. Kinetic model of the biosensors response with consideration of all proteolytic reactions of substrates and products of enzymatic reactions and transport processes in the membranes was elaborated. Conducting polymers and bilayer Upid membranes were used to design sensors and biosensors. 

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