PH effect on enzyme activity

Following these considerations, the pH of these systems was actively controlled by the addition of inorganic salts (acids or bases) [51]. As a matter of fact, a 20-fold increase in the initial rate could be observed upon addition of KHC03 or K2C03 in the synthesis of Z-L-Asp-L-Phe-OMe, but 1.5 equivalents of salt were optimal to obtain yields of more than 90%. The profile of initial rate as a function of K2C03 concentration displayed a bell-shaped figure typically seen for pH effects on enzyme activity (Figure 12.3). 

This book starts with a review of the tools and techniques used in kinetic analysis, followed by a short chapter entitled How Do Enzymes Work , embodying the philosophy of the book. Characterization of enzyme activity reversible and irreversible inhibition pH effects on enzyme activity multisubstrate, immobilized, interfadal, and allosteric enzyme kinetics transient phases of enzymatic reactions and enzyme... 

---