Peroxynitrite pulse studies

Kissner, R., Nauser, T, Bugnon, P, Lye, PG, and Koppenol, WH. 1997. Formation and properties of peroxynitrite as studied by laser flash photolysis, high-pressure stopped-flow technique, and pulse radiolysis. Chem Res Toxicol 10 1285-1292. 

Generation of peroxynitrite by pulse radiolysis involves very tricky adjustments of concentrations of additives and many other experimental conditions. Radiolysis of aerated aqueous solutions containing sodium formate and potassium nitrite in the pH range 3 to 10 produced peroxynitrite according to Eqs. (21) to (23), (40), (41) and (11). For this, initial concentrations of nitrite and formate have to be adjusted in such a way that the radiolytically produced concentration of the radicals of [0 ] -l- [HOj] > NO. However, reactions of peroxynitrite with antioxidants are not generally studied by pulse radiolysis technique. Chemical methods such as ozonolysis of alkaline sodium azide solutions are commonly used to produce peroxynitrite in large quantities and its reactions studied by mixing techniques. 

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