Peroxisome proliferator response element PPRE

Fig. 2. Summary of the mode of action of peroxisome proliferators (PP) through the nuclear peroxisome proliferator-activated receptor (PPAR). The activation of PPAR requires the retinoic X receptor (RXR). The receptors are found in the cytoplasm and once the ligands bind (1) they undergo a conformational change (2) that allows the complex to enter the nucleus (3). The complex then binds to the peroxisome proliferator response element (PPRE) in the promoter region of target genes to alter transcription (4). Modified from Gervois et alP...

Approximately 2.4kb of the promoter region of CTE-I and 2.8kb of the promoter region of MTE-I were sequenced in the 5 direction from the ATG start site. The promoter sequences were analysed for the presence of various transcription factor sites, using TESS String Based Search, which identified putative peroxisome proliferator response elements (PPREs) in both promoters. These regions of the promoters have now been cloned into a luciferase expression vector, to be used in transfection e5q)eriments to examine for functional PPREs. 

Figure 17.2.2. PPARa mediates the rodent response to PPs. Binding sites for PPARa have been found in the promoters of genes associated with peroxisome proliferation such as acyl CoA oxidase, providing proof that PPARa can operate the switch and turn on expression of rodent genes known to be responsive. The binding site within the gene promoter is called a peroxisome proliferator response element (PPREs) and is defined by the DNA sequence TGACCT repeated once with a one letter spacer to give TGACCT n TGACCT.

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