Percentage bound

If, then 100 molecules of A are added, there is a total of 300 molecules of (A + A) competing for 200 binding sites on the antibody (P). Now, when an equilibrium is established, the percentage bound is given by the expression, ... 

If a further 100 molecules of A are added at this stage, the percentage bound shall become ... 

At the dose most commonly employed in the present work (10 mg/kg), 66.6% of phenol red occurred bound as determined by ultrafiltration techniques (8). There were no significant differences in percentage bound at 20 and 40 mg/kg doses whereas there was a slight but significant decrease in the amount of binding by phenol red in the presence of probenecid. 

Percentage Bound = 100 [(total mg IgG added) - (mg IgG in supema-tants)]/(total mg IgG added)... 

By monitoring the percentage bound and/or free radiolabeled antigen as the concentration of unlabeled antigen is increased, one can establish a standard curve. This standard curve can then be used to determine the concentration of an unknown (Fig. 4). 

Which of the following has the least percentage bound to plasma protein a ethanol... 

Fig. 8.5 High-throughput screening at 10mMforWBZ 4 (red) andimatinib (STI 571, blue, control) over a battery of human kinases displayed in a T7-bacteriophage library (Ambit Biosciences, San Diego, CA). Hit values are reported as percentage-bound kinase. Reprinted from the [14], copyright 2007 with permission from the American Society for Clinical Investigation...

Table 3.3 Range of binding of some drugs, at therapeutic concentrations, to plasma proteins in domestic animal species and, for comparison, the average percentage bound in the human.

PERCENTAGE BOUND (g/100g of antioxidant added) BWM MADA... 

Comparison of the data in Figures 3 and 4 shows that while the chemical shift approaches that of the model salt, the linewidth approaches a value one order of magnitude higher. This behavior suggests that Na+ ions in saturated Nafion are substantially restricted relative to the small molecular weight electrolyte, even when the contribution to the linewidth attributable to bound ions is taken into account. This result bears on the theoretical treatment of Nafion dissociation in terms of a four-state model (3). The bulk of the ions appears to be partially restricted (States 2 and 3 of Ref. 3), with a very small percentage bound as contact ion pairs (State 4 of Ref. 3). It is not known if any of the Na+ ions can be considered totally free. 

Many drugs are bound to some extent to plasma proteins. The plasma protein binding is expressed as fraction bound , i.e. the ratio of bound concentration over total (bound plus free) concentration, or as percentage bound if this value is multiplied by 100. Free fraction equals one minus bound fraction. 

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