Peptide mass mapping strategy

The peptide mass mapping strategy using trypsin and V8 protease was applied to solve structural identification problems of the variants. The comparison of the trypsin and V8 protease digest of the native GM-CSF (VO) and... 

Favreau, P., Menin, L., Michalet, S., et al. (2006) Mass spectrometry strategies for venom mapping and peptide sequencing from crude venoms case applications with single arthropod specimen. Toxicon 47, 676-687. 

This strategy requires a more complex system for sample processing however, it engages the advantages of improved mass accuracy and better sensitivity that derive from working with lower mass ions. Peptide maps from protein mixtures have been demonstrated to provide reliable identifications of microorganisms that are reasonably pure.79-81... 

An approach similar to that used for the study of 3D structures can also be used to detect protein-protein interactions.166 Two strategies have been developed for this purpose bottom-up and top-down. In the former approach, the protein complex is cross-linked, as mentioned earlier, and digested proteolytically the complete peptide map is then analyzed by mass spectrometry to provide the identity of the cross-linked peptides. In the... 

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