Penidllin G acylase

Ospina S, Lopez-Munguia A, Gonzalez R et al. (1992) Characterization and use of a penicillin acylase biocatalyst. J Chem Technol Biotechnol 53 205-214 Ospina S, Barzana E, Ramirez O et al. (1996) Effect of pH in the synthesis of ampicilfinby penicillin acylase. Enzyme Microb Technol 19 462-469 Palazzi E, Converti A (2001) Evaluation of diffusional resistances in the process of glucose isomerization to fructose by immobilized glucose isomerase. Enzyme Microb Technol 28 246-252 Pan JL, Syu MJ (2004) A thermal study on the use of immobilized penidllin G acylase in the formation of 7-amino-3-deacetoxy cephalosporanic acid from cephalosporin G. J Chem Technol Biotechnol 79(10) 1050-1056... 

Wang J, Zhang Q, Huang H et al. (2007b) Increasing synthetic performance of penidllin G acylase from Bacillus megaterium by site-directed mutagenesis. Appl Microbiol Biotechnol 74 1023-1030... 

In Bgure 6.11 we indicated that penidllin acylases selectively hydrolysed the secondary amide link, releasing 6 aminopenidllanic add (6-APA). Although these enzymes could be used to produce 6-APA from penicillin G, initially, the vulnerability and hirfi costs of enzymatic deacylation were impcnlant reasons to search for alternative, diemical processes. 

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