PEI-pyridoxamine

The PEI-pyridoxamine polymer was treated with excess pyruvic acid in various buffer solutions, without added metal ions and with excess added EDTA. Kinetic studies revealed that the attached polymer increased the rate of pyridoxamine transamination with pyruvic acid by a factor of6700-8300 at pH 5.0. Under higher pH conditions, the rate enhancement decreased At pH 7.0 the rate enhancement by the polymer was still 2300 times, while at pH 8.0, the optimum for pyridoxamine itself, it was 1900. We also found that transamination by simple pyridoxamine showed strong metal ion catalysis - adding 1 equiv. of CuCl2 per pyridoxamine unit to the pH 5.0 solution increased the... 

Figure 2.5 Structure of the PEI-pyridoxamine systems. (Reprinted from Ref. 36. Copyright 2002 American Chemical Society.)...

---