Pectinesterase distribution

Rouse, A.H. 1953. Distribution of pectinesterase and pectin in component parts of citrus fruits. Food Technol. 7 360-362. 

Miller and Macmillan [4] carried out purification of pectinesterase from Fusarium oxysporum f. sp. vasinfectum culture fluid (fivefold degree of purification). According to the obtained data the purified enzyme possessed very low polygalacturonatlyase one. Disk electrophoresis at pH 4.3 revealed two protein components. The authors did not study distribution of pectinesterase activity in these components. Molecular weight of fungal pectinesterase determined using gel — filtration on Sefadex G — 75 was found to be 35,000. 

Fig. 28. Change in reduced viscosity and scattered light during addition of CaCl2 to pectins (polsmier concentration 0.2 g/L 5 x 10 M CaCl2). Role of the carboxyl groups distribution (a) DE = 30% blockwise distribution obtained by pectinesterase hydrolysis (b) DE = 30% random distribution obtained by alkaline hydrolysis. Reproduced from Ref 164, with permission from John Wiley Sons, fric., on behalf of the Society of Chemical Industry (Copsright SCI 2003).

---