Peak heights/areas

Minimum peak height/area Lets the computer know the minimum peak height or area below which the computer may disregard the peak (eliminates noise). 

If the threshold and minimum peak heights/areas were appropriately defined, then only peaks 1 and 7-12 would be integrated, making the report much easier to read. 

Simple, robust quantitative algorithms (peak height/area of isolated bands) standards usually needed... 

Data supplied Peak height Peak height, area and width peak-to-peak distance... 

For most applications, this is all the sample preparation that is necessary. Appropriate dilution is deceptive as it will vary from one commodity to another dependent on the number of anthocyanins in the matrix. The visual appearance and absorptivity of a sample is often not a sufficient indicator for HPLC peak height/area, as samples that have many anthocyanin peaks will need to be more concentrated than a sample that has the same total anthocyanin content but only a single major peak. Most anthocyanin-containing fruit juices will give a suitable response between single strength juice and a dilution of 1 5. 

In the absence of chemical reactions (such as in a simple atomic absorption measurement) when the detector responds linearly and instantly to the injected species, it does not make any difference whether peak height, area, or peak width is being measured, since they all yield useful information (Fig. 2Ab), although the concentration of the injected material is related to each of these parameters in a different manner. The same comments apply to assays based on production of a measurable species (such as in spectrophotometry) provided that via the dispersion process an excess of reagent is available throughout the entire length of the dispersed sample zone. (For exceptions see Section 2.4.4.)... 

Fig. 25. The dependence on the elapsed time of the ratios of the peak heights areas under the peaks H2/H, in the temperature regions 180-300 and 25-180, for the CaS04 Dy and CaS04 LiF, Dy phosphors.

A technique called least squares regression is used to solve for the model equation using the peak height/area data and the known constituent quantities. This mathematical technique calculates the coefficients of a given equation such that the differences between the known spectral responses (peak areas or heights) and the predicted spectral responses are minimized. (The predicted spectral responses are calculated by reading the measurements off the calibration line at the known concentrations see Fig. 1.) As... 

Standardize injection volumes for more reproducible results, since change in injection volume will influence the retention time and hence peak height area ratio. If peak areas are used for quantitation instead of peak heights, changes in injection volume have little effect. 

Peak height/area has LITTLE MEANING in C-13 NMR. Peak area is a function of many things, one being whether the C is 1°, 2°, 3°, or 4°. It is difficult to interpret the meaning of peak height in C-13 NMR... 

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