Peak, asymmetry optimized column

The responses of main interest are different during both applications. In optimization, responses related to the separation of peaks (Section 6.2) are modelled. In robustness testing the quantitative aspect (the content determination) of the method is of most interest, since it is the one that should remain unaffected by small variations in the variables. Responses related to the separation (resolution, relative retention) or describing the general quality of the chromatogram (capacity factors, analysis times, asymmetry factors, and column efficacy) are often also studied. As recommended by the ICH guidelines the results of a robustness test can be used to define system suitability test limits for some of the responses [82]. 

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