PBN adduct in coronary effluents during reperfusion

Detection of PBN adduct in coronary effluents during reperfusion 

The ESR spectra of the toluene extract of the coronary effluents collected during aerobic reperfusion are shown in Figs. 7a-e. The signal intensity increased during the early minutes of reperfusion and then declined. The ESR hyperfine parameters were calculated to be about on = 14.0 0.1G and a = 2.0 0.1 G. No ESR spectra were detected from the aqueous coronary effluents under otherwise identical conditions. Extraction with toluene was, therefore, absolutely crucial to detect an ESR signal from the PBN-adduct. At lower temperatures ( —100°C), the ESR signal intensity was increased with a slight loss in spectral resolution. Similar results have previously been reported [128]. 

---