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Parus bicolor

Fig. 2. Effect of DNA concentration and number of PCR cycles on RAPD analysis, shown on ethidium bromide-stained agarose gels, using template DNA from a single individual titmouse (Parus bicolor) and the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). (A) Various concentrations of template DNA amplified through 45 cycles of PCR. Lanes 1-11 contain 100, 50, 20, 10, 5, 2, 1, 0.1, 0.05, 0.01, and 0.005 ng of template DNA, respectively, in 25-/ Fig. 2. Effect of DNA concentration and number of PCR cycles on RAPD analysis, shown on ethidium bromide-stained agarose gels, using template DNA from a single individual titmouse (Parus bicolor) and the 10-base primer AP5a4 (5 CTGTTGCTAC 3 ). (A) Various concentrations of template DNA amplified through 45 cycles of PCR. Lanes 1-11 contain 100, 50, 20, 10, 5, 2, 1, 0.1, 0.05, 0.01, and 0.005 ng of template DNA, respectively, in 25-/<l reactions. Lane 12 contains no template DNA. (B) Constant amount of template DNA (0.6 ng/id) amplified with primer AP5a between 20 and 45 cycles. All reagents for the experiment were combined in a single tube, then aliquoted into twelve 25-/d reactions. Duplicate reactions were performed for each cycle length variation.
Forster s ten. Sterna forsteri, 265, 266, 632, 642 Little tem. Sterna albifrons, 436 Sooty tem. Sterna fuscata, 439 Ht, great, Pams major, 254, 631 Titmouse, tufted, Parus bicolor, 254 Thrush... [Pg.942]

See other pages where Parus bicolor is mentioned: [Pg.1008]    [Pg.1008]    [Pg.300]    [Pg.307]    [Pg.254]    [Pg.1008]    [Pg.1008]    [Pg.300]    [Pg.307]    [Pg.254]   
See also in sourсe #XX -- [ Pg.1005 ]

See also in sourсe #XX -- [ Pg.1005 ]



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Bicolor

Parus

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