Parallax Quenching in Membranes

Lii is the distance beMroen die sh low ud deep qu cb ers. The shallow quencher is located at a distance La from the center of the bilayer. Tliese distances are shown ia IRgure 9.12. C is the concentration of quenches in mde-cules per unit area. Fi and F2 are the relative intensities of the fluorophore in the presence of the shallow and the deep quencher, respectively. This model was derived by assume ing that quenching is complete within acridcal distance of the quencher and that quendiing does not occur across the bilayer. This model also assumes dial there is no diffusion in the membrane. 

F%ure9.13. QnendtingofNBD-labdedUfndimmembranescontain fine of tbaec qiin-Iabeled (SL) PCs. Sec Hguie 9.6 the prc stnictuies. Remn Ref. 31. 

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