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Pantothenate agar

Growth on pantothenate agar, a culture medium containing no pantothenic acid (BF positive)... [Pg.71]

Figure 2. Effect of ATP on pantothenic acid synthetase activity of frozen-thawed cells immobilized in agar. The experiment was carried out at 30°C and... Figure 2. Effect of ATP on pantothenic acid synthetase activity of frozen-thawed cells immobilized in agar. The experiment was carried out at 30°C and...
Flooding trikaryons After 3 days of incubation at room temperature the tubes are flooded with Fries minimal + 10 mg/liter calcium pantothenate + 0.75% fructose + 0.75% glucose + 1.2% agar. This flooding medium should be autoclaved and cooled to 45°C. It is then dispensed, 2 ml per tube, with a buret. Each tube is slanted after flooding and then incubated at room temperature for 5-7 days. [Pg.41]

Plating trikaryons After the trikaryons have conidiated they are plated on Fries minimal + 10 mg/liter calcium pantothenate + 1.0% sorbose -f- 0.05% glucose + 0.05% fructose + 1.5% agar, dispensed 12 ml into 20 by 150 mm test tubes. The trikaryons are plated into divided 15 by 100 mm petri plates as discussed for dikaryons. [Pg.41]

See other pages where Pantothenate agar is mentioned: [Pg.72]    [Pg.76]    [Pg.79]    [Pg.72]    [Pg.76]    [Pg.79]    [Pg.72]    [Pg.76]    [Pg.79]    [Pg.72]    [Pg.76]    [Pg.79]    [Pg.134]    [Pg.135]    [Pg.138]    [Pg.39]    [Pg.820]   
See also in sourсe #XX -- [ Pg.76 , Pg.79 ]

See also in sourсe #XX -- [ Pg.76 , Pg.79 ]



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