Palmostatins Inhibit Depalmitoylation of Ras GTPases

Microscopy on single cells using proteins that are fluorescently labeled. Fluorescent protein labels that are often used are yellow fluorescent protein (YFP), GFP, or Citrine. Fluorescent chemical labels are Cy3 and Cy5. This technology can be used for steady-state studies or for time-resolved studies. For time-resolved studies, proteins are microinjected or photoactivated/bleached and fluorescence changes are monitored overtime. 

Microinjection is a process in which very fine needles are used to inject substances into a single living cell under the microscope. Small needles of roughly 0.5-5 pm in diameter are used to inject the desired contents into the desired subcellular compartment. 

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