Packed columns kinetic optimization

The temperature profile in a CD column is controlled by column pressure and is not isothermal. The kinetics of the reaction should be known prior to the CD experiments so that the CD packing could be located in the section of the column where the temperature is optimal for production of a specified product. 

The effect of pore size of the packing on the mass transfer kinetics of biopolymeric solutes in HPLC column separations has been a subject of intensive studies (as an example see Ref 27). To optimize the mass transport properties of the supports, the focus was directed to packings with a bimodal pore size distribution having mesopores (diffusional pores) and macropores (transport pores). This has led to a new design of porous packings suitable for fast biopolymer separations at a preparative scale that exhibit a high dynamic capacity for biopolymers [28,29]. 

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