P75 co-receptor

Fig. 13. A model for initiation of NgR signaling. While NgR exists primarily as a monomer in solution, studies have shown both self association and interactions with the co-receptor p75 on the cell surface. Currently, the stoichiometry of ligand and co-receptor binding is unknown.

Fig. 2. TrkA associates with p75 receptor in 293T cells. 293T cells ( 1 X 10 ) were co-transfected with p75 and either TrkA or empty vector. 36 h after transfection, the cells were harvested into TNE buffer [10 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, and 1% NP40], The lysates were immunoprecipitated with anti-pan Trk antibody (Cl4, Santa Cruz) which recognizes the C-terminus of Trk and subsequently subjected to Western blot analysis with an anti-p75 antiserum (9992), or anti-pan Trk antiserum. The amount of p75 protein for each transfection is assayed in the bottom panel. The protein visualized in both lanes of the top panel reflects reactivity of the heavy chain of IgG.

To address the state of endogenous TrkA and p75 receptors in PC 12 cells, co-immunoprecipitation experiments have been carried out in PC 12 cells. A subline of PC 12 (615) cells that expressed higher levels of TrkA receptors (Hempstead et al., 1992) was employed. The PC12 615 cell line was subjected to NGF treatment for 10... 

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