Oxygenated trichodiene

T-2 toxin is greatly inhibited. Again, there is molecular specificity because only one of fifteen flavonoids tested and eleven of twelve furanocoumarins inhibited toxin production (26). But when inhibition was induced, trichodiene (a trichothecene precursor) increased proportionately and the sequential biosynthetic steps in which oxygenation of the molecule normally occurs did not take place. 

Much effort, using mainly Fusarium spp., has been devoted to the identification of the oxygenation, cyclisation and esterification steps which take place after the formation of trichodiene. Fermentations carried out on a large scale, or in the presence of enzyme inhibitors, such as ancymidol (225) or xanthotoxin (219), or with mutant strains (223), have yielded metabolic products [(47), (52), (53), (59) Table 8] which are proven intermediates. They are derived from trichodiene by plausible pathways involving allylic hydroxylation at positions 2a and 11a, fol-... 

Zamir LO, Nikolakakis A, Huang L, St-Pierre P, Sauriol F, Sparace S, Mamer O (1999) Biosynthesis of 3-Acetyldeoxynivalenol and Sambucinol. Identification of the Two Oxygenation Steps After Trichodiene. J Biol Chem 274 12269... 

Zamir LO, Devor KA, Morin N, Sauriol E (1991) Biosynthesis of Trichothecenes Oxygenation Steps Post-trichodiene. Chem Commun 1033... 

Zamir LO, Devor KA, Morin N, Sauriol F (1991) Biosynthesis of trichothecenes oxygenation steps post-trichodiene. J Chem Soc Chem Comm 1033-1034 McCormick SP, Taylor SL, Plattner RD, Beremand MN (1990) Bioconversion of possible T-2 toxin precursors by a mutant strain of Fusarium sporotrichioides NRRL 3299. Appl Environ Microbiol 56 702-706... 

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