Oxygenase-nitrilase

Environmental application for bioremediation purposes must also get the benefit of the technology. Since haloalkane dehalogenase activity was tested successfully, as shown in Example 2 above for the dehalogenation of toxic VOCs, many others enzymatic activities should be of great interest, such as oxygenases, nitrilases, and organophosphorus hydrolases, for example. 

These compounds are L-dopa (13-tyrosinase) D-p-hydroxyphenylglycine, D-phenylglycine (hydantoinase) ethyl (R)-4-chloro-3-hydroxybutanoate (aldehyde reductase) acrylamide, nicotinamide (nitrile hydratase) acrylic acid, nicotinic acid (nitrilase) 6-hydroxynicotinic acid (hydroxylase) D-malic acid (maleate hydratase) D-pantoic acid (aldonolactonase) and theobromine (oxygenase). 

Nitriles have a broad range of applications as building blocks. Biocatalytic conversion of nitriles [40] has been performed by nitrilases, nitrile hydratases, nitrogenases, and oxygenases so far, leading to an ample number of molecules... 

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