Overpressured layer chromatography stationary phase

The second example relates to the separation and determination of steroid isomers. The separation of R- and S-budesonide, syn- and antinorgestimate, -norgestreloxime, and 6-hydroxy-norgesti-mate have been done on silica, and amino-bonded silica stationary phases by conventional and overpressurized layer chromatography (OPLC) (35). Application of amino-bonded silica as stationary phase, and chloroform-diethyl ether-methyl ethyl-ketone (4 3 3, v/v) as mobile phase results in a satisfactory separation of the isomer pairs, especially, if OPLC technique is used. 

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