Other Compounds containing the C—F Link

OTHER COMPOUNDS CONTAINING THE C-F LINK Amines, as might be expected, take the following course F[CHj],CH H,---- F[CHj],CH=NH ---- FlCH,]sCHO --> F[CH,]jCOOH. 

In this work on compounds containing the C—F link, it was obviously desirable to prepare 2-fluoroethanol, both for toxicity tests on the compound itself, and as a starting material for the production of other fluorine compounds. Swarts1 was unable to obtain 2-fluoroethanol by the action of silver fluoride or mercuric fluoride on either ethylene chlorohydrin or ethylene bromohydrin. He obtained acetaldehyde in each case. He ultimately obtained fluoroethanol in very poor yield by the indirect method of hydrolysing fluoroacetin (from bromoacetin and mercuric fluoride) for 80 hr. with dilute mineral acid. 

Figure 5 Schematic representation of the most widely used noncompetitive biologieal immunoassay, the enzyme-linked immunosorbent assay (ELISA), (a) Antibody, selective for analyte, is immobilized on microtiter plate well surface (b) sample added (c) analyte in sample binds to antibody while other compounds in matrix remain in solution (d) sample solution containing nonbound molecules discarded and wells washed (e) analyte remains bound to antibody (f) second antibody nzyme conjugate added (g) conjugate binds to bound analyte (h) solution containing nonbound conjugate discarded and wells washed (i) conjugate remains bound 0 enzyme substrate added (k) substrate S converted to colored or fluorescent product P at a rate which is proportional to the amount of bound enzyme and hence to the concentration of analyte in the sample.

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