Ot-l.-Fucosidase

H. Hashimoto, K. Shimada, and S. Horito, Synthesis of 1-6, 1-4 and 1-3 linked lthio-a-1-fucopyranosyl-2-acetamido-2-deoxy-P-D-glucopyranosides and 1-2 linked p-o-galactopyran-oside, and their linkage-specific inhibitory activities toward ot-L-fucosidases, Tetrahedron Lett., 34 (1993) 4953-4956. 

Traces of ot-L-fucosidase activity have been found in axenic cultures of the myxo-mycete Physarum polycephalum. ... 

The ot-L-fucosidase from the marine mollusc Littorina littorea has been purified only one form was detectable by isoelectric focusing at pH 5.4. L-Fucose was a competitive inhibitor and Hg + ion was a non-competitive inhibitor of the enzyme (mol. wt. 2.2 x 10 , pH optimum 6.5, Km 3.0 x 10 M and Fmax 2.6 (xmol mg min for 4-nitrophenyl a-L-fucopyranoside). The enzyme proved to be a glycoprotein containing D-glucose (7.4), D-galactose (2.2), D-mannose (1.8), and 2-amino-2-deoxy-D-hexose (2.2%). 

---