Organ cultures

Organ cultures. Differentiated tissues of shoots, roots, anthers, ovaries, or other plant organs in culture... 

A method of assessing the toxicity of implants has been proposed based on the effects on cell ultrastructure in organ cultures, on cell surface characteristics, and cell population doubling times. The effects have been correlated with hemorrhage, fibrosis, and necrosis, respectively (103). Poly-e-caprolactone was stated to give minimal tissue reaction and could not be scored in these tests. 

Phaseolus vulgaris isolectins on pig jejunal mucosa in organ culture. Gut. 32 886-92. 

NPYR NNN, NPIP D-NPZ Human Colon organ culture metabolism to CO2 and binding to DNA and protein 28... 

Uchiyama T., Numata M., Tereda S., Hosino T. Plant cell Tissue and Organ Culture 32 ... 

Uchiyama T., M. Numata, S.Terada, T. Hosino, Plant Cell Tissue and Organ Culture 32 (1993) 153. 

Wink M, Plant Cell Tissue and Organ Culture 38 (1994) 307. 

Brook, I. M., Craig, G. T. Lamb, D. J. (1991a). In vitro interaction between primary bone organ cultures, glass-ionomer cements and hydroxyapatite/tricalcium phosphate ceramics. Biomaterials, 12, 179-86. 

Hetem, S., Jowett, A. K. Ferguson, M. W. (1989). Biocompatibility of a posterior composite and dental cements using a new organ culture model. Journal of Dentistry, 17, 155-61. 

Mossman, B.T., Craighead, J.E. and MaePherson, B.V. (1980). Asbestos-induced epithelial changes in organ cultures of hamster trachea inhibition by retinyl methvi ether. Science 207, 311-313. 

Functional TRPVl is expressed in keratinocytes, hair follicles, sebocytes and sweat gland cells [151, 152]. In organ cultures, activation by capsaicin of... 

In some cases, cell culture monolayers can not be obtained but whole organs, or pieces of organs, can be cultured. Such organ cultures may still be useful in virus research, since they permit growth of viruses under more or less controlled laboratory conditions. 

Sugg and Hehre43 also obtained precipitin reactions with dextran or with sterile filtrates of sucrose broth cultures of L. mesenteroides (designated for convenience strain A) and not only anti-Leuconostoc sera, but also pneumococcus Types II, XII and XX antisera. Leuconostoc organisms cultured on D-glucose broth neither stimulated the production of dextran-reactive antibodies in rabbits, nor absorbed dextran-reactive antibodies from sera, as did organisms cultured on sucrose. Absorption with the homologous bacteria (Leuconostoc, pneumococcus Types II,... 

In BA metabolism, the procarcinogenic BA trans-3.4-dihydrodiol (26) constitutes 1.5-4% of all the metabolites formed by rat liver microsomes (27) and a major component of the free dihydrodiols formed by mouse skin maintained in short-term organ culture (28). In this system (28). the noncarcinogenic dihydrodiols may be preferentially removed by conjugation reactions to yield water soluble products. 

Bohn, M. C., Bloom, E., Goldstein, M. and Black, I. B. Glucocorticoid regulation of phenylethanolamine N-methyl-transferase (PNMT) in organ culture of superior cervical ganglia. Dev. Biol. 105 130-136,1984. 

Ryther, J., T.M. Losordo, A.K. Furr, T.F. Parkinson, W.H. Gutenmann, I.S. Pakkala, and D.J. Lisk. 1979. Concentration of elements in marine organisms cultured in seawater flowing through coal-fly ash. Bull. Environ. Contam. Toxicol. 23 207-210. 

Lai, Z., et. al., Differential effects of diethylstilbestrol and 2,3,7,8-tetrachlorodibenzo-p-dioxin on thymocyte differentiation, proliferation, and apoptosis in bcl-2 transgenic mouse fetal thymus organ culture, Toxicol. Appl. Pharmacol., 168, 15, 2000. 

Haul At 24 h in organ culture we find that RyR2 has been up-regulated by a factor of two or three. 

Hellstrand With regard to the question of what ryanodine is doing, if you put it onto a muscle it eliminates most SR Ca2+ release, because it opens up the SR Ca2+ channels and there is some level of communication between the stores. If you put ryanodine there you essentially kill InsP3-induced responses as well. In organ culture we have found that if you culture in the presence of ryanodine for a couple of days, the InsP3-induced release reappears, whereas there is still no ryanodine-induced release, and Ca2+ waves occur (Dreja et al 2001). We know that mitochondrial activity affects the properties of Ca2+ waves, so there is a Ca2+-dependent modulation of SR release on this level which thus presumably involves InsP3 receptors and not RyRs. It is very hard to distinguish these two receptor populations in the way they interact on the SR. 

Giri A, Dhingra V, GiriCC, Singh A, Ward OP, Narasu ML (2001) Biotransformations using plant cells, organ cultures and enzyme systems current trends and future prospects. Biotechnol Adv 19 175-199... 

Asakura, T., Sakaguchi, R., Demura, M., Manabe, T., Uyama, A., Ogawa, K., and Osanai, M. (1993). In vitro production of Bombyx-mori silk fibroin by organ-culture of the posterior silk glands—isotope labeling and fluorination of the silk fibroin. Biotech-nol. Bioeng. 41, 245-252. 

Several tetracyclines with collagenase inhibitory activity have also been shown to inhibit parathyroid-induced bone resorption in organ culture [229]. Subsequent experiments with CI-1 have reinforced the view that a collagenase is involved in bone resorption and, in fact, the resorption of demineralized collagen by collagenase may be the rate-limiting step [230,231 ]. 

The periodontal pocket is another site for drug delivery in the oral cavity. Needleman et al. [46] investigated three mucoadhesive polymers (cationic chitosan, anionic xanthan gum, neutral polyethylene oxide) in vitro, using organ cultures, and in vivo in patients on their periodontal and oral mucosa. Of the polymers studied, chitosan displayed the longest adhesion in vitro and on the periodontal pockets, and the shortest adhesion on oral mucosa. 

Golub NI. 1970. [Transplacental action of 3,3 -dichlorobenzidine and ortho-tolidine on organ cultures of embryonie mouse kidney tissue.] Bull Exp Biol Med 54 1280-1283. [Russian]... 

Shabad LM, Sorokina JD, Golub NI, et al. 1972. Transplacental effect of some chemical compounds on organ cultures of embryonic kidney tissues. Cancer Res 32(3) 617-627. 

Leaf organ cultures of C. roseus have also been described 118). A typical 2.5-g fresh weight inoculum produced 29 g fresh weight of leaf material after 35 days dedifferentiated tissue was absent. The alkaloids found included ajmalicine (39), sitsirikine (48), tetrahydroalstonine (39), serpentine (40), and vindoline (3). 

Previously, hver shces were incubated in static organ cultures [1]. Hart et cd. [49] cultured rat hver slices for 24 h spread out on wet filter paper, floating on top of the incubation medium. Several slice-containing vessels were placed in a box with saturated 95% O2 and 5% CO2 at 37°C. However, the shces employed were rather thick (approximately 0.3 mm) and only the upper cell layers (0.2 mm) in the slice contained viable ceUs. Together with the introduction of the Krumdieck sheer [5,46], a new incubation technique for shces, the dynamic organ culture system (DOC), was introduced [35]. The main characteristic of this system is the intermittent exposure of the shce to incubation medium and the gas phase. The DOC is in fact a modified version of the Trowell incubation system [1]. 

---