Optimization of a chromatographic analysis

Analytical chromatography is used essentially in quantitative analysis. In order to achieve this effectively, the areas under the peaks must be determined with precision, which in turn necessitates well-separated analytes to be analysed. A certain experience in chromatography is required when the analysis has to be optimized, employing all available resources in terms of apparatus and software that can simulate the results of temperature modifications, phases and other physical parameters. 

If only certain compounds present in a mixture are of interest, then a selective detector can be used which would detect only the desired components. Alternately, at the other extreme, attempts might be made to separate the largest number of compounds possible within the mixture. 

Depending upon the different forms of chromatography, optimization can be more or less rapid. In gas phase chromatography optimization is easier to achieve than in liquid chromatography in which the composition of mobile phase must 

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