Optimisation Strategies in Polyhydroxyalkanoate Fermentation

Fed-batch cultivation is a fermentation strategy whereby one or more nutrients are supplied to the cell culture until the end of the process. The concentration of nutrients that are fed into the bioreactor can be controlled by regulating the feed rate. In fed-batch cultivation the cells are fed continuously as per the required feeding method regime 

There are two types of fed-batch production one results in a growth-associated product and the second results in a non-growth associated product. Cupriavidus necator has been reported to produce a nongrowth-associated product. In this type of production, there is a microbial growth phase followed by a PHA production phase 

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