Optimal Handling and Preservation Methods

The stability of the biomarker over different temperatures and times may vary based on the biological matrix. For some biomarkers, it may be necessary to add preservatives, stabilizers, and/or buffers for biomarker stability. For studies where multiple biomarkers will be analyzed, it may be necessary to process the samples using separate, biomarker-specific 

During assay validation the optimal storage preservation tubes should also be determined. Low binding mbes may be preferred for optimal recovery of some analytes. Sample size/aliquot volume should be sufficient for the downstream assay requirements, because freeze/thaw may compromise sample stability (see Chapter 41 page 483) and multiple amounts/aliquots may be needed. The number of samples has to be balanced with storage costs and availabihty. 

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