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Optical enzyme-based sensors immobilization process

A DNA optical sensor system was proposed by Cass and co-workers [35] based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for the rapid detection of DNA hybridization. Sandwich solution hybridization uses two sets of DNA probes, one labelled with biotin, the other with an enzyme marker and hybridization is performed in solution where the mobility is greater and the hybridization process is faster, rather than on a surface. The hybrids were bound to the streptavidin-coated magnetic beads through biotin-streptavidin binding reaction. A chemiluminescence fibre-optic biosensor for the detection of hybridization of horseradish peroxidase-labelled complementary DNA to covalent immobilized DNA probes was developed by Zhou and co-workers [36]. [Pg.388]


See other pages where Optical enzyme-based sensors immobilization process is mentioned: [Pg.164]    [Pg.15]    [Pg.303]    [Pg.87]    [Pg.114]    [Pg.1278]    [Pg.378]    [Pg.264]    [Pg.447]    [Pg.316]    [Pg.5710]    [Pg.220]    [Pg.418]    [Pg.395]    [Pg.400]   
See also in sourсe #XX -- [ Pg.61 , Pg.62 ]




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Enzyme immobilization

Enzyme processes

Enzyme processive

Enzyme sensors

Enzyme-based sensor

Immobilized enzymes

Optical Processing

Optical enzyme sensors

Optical processability)

Optical processes

Optical sensors

Process optical sensors

Sensors based

Sensors process

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