One-site immunometric assay

In a one-site immunometric assay, the sample is first incubated with a known excess of labeled antibodies (or Fab fragments) that are specific for the analyte of interest. After this binding has occurred, the mixture is applied to a column that contains an immobilized analog... 

The technique of postcolumn immunodetection involves the use of an lAC column that is attached to the exit of an analytical HPLC system. The lAC column in this approach serves to collect and retain a specific analyte from the HPLC column eluent for later detection. Both the on-off mode and immunoassay formats of I AC have been used as strategies for postcolumn immunodetection. The most common of these approaches is the one-site immunometric assay. One reason for this is that the immobilized analog columns in one-site immunometric assays often have a much more flexible selection of elution conditions than immobilized antibody columns. Another reason is that one-site immunometric columns can usually be used for many sample injections before they are eluted and regenerated, which helps to decrease the overall analysis time associated with their use in postcolumn detection. 

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