Omithopus

Omithopus sativus (seed, shoots) Leguminosae Castasterone (3), 6-Deoxocastasterone (10), 24-Epicastasterone (13), 6-Deoxo-24-epicastasterone (33), 6-Deoxo-28-norcastasterone (34) 47,48... 

Two new brassinosteroids could be detected from the shoots of Omithopus sativus (see. Section 2). One of them, 6-deoxo-24-epicastasterone (33), was synthesized by us from 24-epicastasterone (13) via the corresponding thioacetal 70 and subsequent reductive elimination of the thioketal group by reaction with tri-n-butyltin hydride (BujSnH) in the presence of 2,2 -azabis-2 -methylpropionitrile (A1BN) (Scheme 8).48... 

Ri Cholesterol (C27) pY k°" 28-Nurcastasterone VsY Rs 28-Norbrassinolide Zea mays llen) [21] Omithopus sativus ( oot)f22] Lycopercicon esculentim (shoot) [23] (10 species)... 

Metabolism of 24-epicastasterone and 24-epibrassinolide in cultured cells of tomato and Omithopus sativus... 

Fig. 5. Metabolism of 24-epicastasterone in the cultured cells of tomato and Omithopus sativus. Identified as a glycoside(s) in tomato cells Identified as the free form in Omithopus cells...

In Omithopus sativus cells, a to p epimerization of the 3-hydroxyl is quite important in the metabolism of 24-epicastasterone (Fig. 5) and 24-epibrassinolide (Fig. 6) because all the metabolites have a 3p-hydroxyl group [74-76], Thus 3,24-diepicastasterone is formed first and then metabolized through two different pathways which are not observed in tomato cells (Fig. 5). One is the acylation of the C3p-hydroxyl leading to a mixture of... 

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