Oligonucleotide Raman spectra

Fig. 4. The Raman spectrum of crystals containing the oligonucleotide d(TCGCGAATTCGCG) in the B form bound to the restriction endonuclease Eco Rl (Top spectrum). The Raman spectrum of crystals of the restriction endonuclease Eco Rl in the absence of oligonucleotide (Bottom spectrum) [89T1]. For clarity of the figure the top spectrum is displaced...

An attempt to record the SERS signals of the oligonucleotide d(CG)g resulted in an uninformative spectrum (Fig. 20.13, top). In spite of the comparatively high total concentration (2 mg/ml 3 mM), the signal-to-noise ratio is poor and only a few vibrations that are characteristic for d (CG)8 are detected. In the bottom trace, the normal Raman spectrum recorded at a 10 times higher concentration is reproduced from Fig. 20.10 for comparison. 

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