Oligodeoxyribonucleotides, synthesis, phosphotriester method

Sproat BS, Gait MJ. Solid-phase synthesis of oligodeoxyribonucleotides by the phosphotriester method. In Gait MJ, ed. Oligonucleotide Synthesis A Practical Approach. Oxford IRL Press Ltd, 1984 83-115. 

MSNT has been employed as the condensing agent in a conti-nuous-fiow, solid-phase synthesis of oligodeoxyribonucleotides using the phosphotriester method (eq 2). ... 

The phosphotriester method of synthesis may be regarded as the method of choice for the large-scale preparation of oligodeoxyribonucleotides. Although this method is perhaps not so readily adaptable to... 

Gait, M. J, Matthes, H W. D., Singh, M., Sproat, B S., and Titmas, R. C. (1982) Rapid synthesis of oligodeoxyribonucleotides VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support. Nucl Acids Res. 10, 6243-6254. 

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