Oligodeoxynucleotides purification

Primer quality is important. If the amplification reaction is initiated with a primer containing significant levels of premature termination products, the RAPD reaction will usually not work. If care is taken during the synthesis and storage of the oligodeoxynucleotide, purification is usually not required beyond deprotection and desalting of the primer. 

Fig. 7. Semi-Preparative Anion Exchange Purification of a 16-mer Oligodeoxynucleotide on a CIM DEAE Disk Monolithic Column. Conditions Column 0.34 ml CIM DEAE Disk (3X12 mm ID) Instrumentation Gradient HPLC system with extra low dead volume mixing chamber Sample 16mer oligodeoxynucleotide from the reaction mixture - bold line, standards of 1,2,3,4,5,6,7,9,10,11,12,14,15,16mer- thin line Injection Volume 20 pL Mobile Phase Buffer A 20 mM Tris-HCl, pH 8.5 Buffer B Buffer A+ 1 M NaCl Gradient as shown in the Figure Flow Rate 4 ml/min Detection UV at 260 nm...

Jones, R A., Fritz, H J., and Khorana, H G. (1978) Studies on polynucleotides 147 Use of the lipophilic rerr-butyldiphenylsilyl protecting group in synthesis and rapid separation of polynucleotides. Biochemistry 17, 1268-1278 Mishra, R. K. and Misra, K. (1988) Protecting groups as purification tool in large-scale synthesis of small oligodeoxynucleotides Indian J, Chem Sect. B, 27B, 817-820. 

Biernat, J, Wolter, A., and Koster, H. (1983) Purification oriented synthesis of oligodeoxynucleotides in solution. Tetrahedron Lett, 24,751-754... 

Purification and analysis of oligodeoxynucleotides synthesized on solid phase supports... 

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