Oligo- and Polynucleotide Synthesis

In the phosphotriester synthesis, 5 -0-MMTr-((V-acylated)-deoxyribonucleotide-3 -0-(CP phosphate) is coupled to a 3 -hydroxyl protected deoxyribonucleotide or an existing chain using mesitylenesulfonyl chloride as the coupling agent. 

In the phosphite triester synthesis (phosphoramidite synthesis), an efficient coupling reaction takes place between the 3 -hydroxyl group of the chain and 5 -DMTr-(A -acy-lated)-deoxyribonucleoside 3 -0-(MA -diisopropylamino)phosphite. 

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