Of D-fructose 6-phosphate

When D-glucose 6-phosphate and D-fructose 6-phosphate are inter-converted by D-glucose 6-phosphate ketol isomerase in either deuterium oxide33 or water-1 (Ref. 34), isotope is incorporated at C-l of D-fructose 6-phosphate and C-2 of D-glucose 6-phosphate, indicating that the interconversion involves an enediol intermediate, which may arise from an open-chain (37) or cyclic (38) form of the sugar,... 

S466E <3> (<3> mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme in vitro [63]) [63] S466E/S483E <3> (<3> the double mutant is not phosphorylated by protein kinase B, mutation decreases Km of -D-fructose 6-phosphate in vitro [63]) [63]... 

Prokaryotic organisms synthesise a primary form of vitamin pyridoxol 5 -phosphate from 1-deoxy-D-xylulose 5-phosphate (l-deoxy-D-t/treo-pent-2-ulose 5-phosphate) and 2-amino-2-deoxy-D-threo-tetronic (2-amino-2-deoxy-D-threonic) acid, known as 4-(phosphohydroxy)-L-threonine or 4-hydroxy-i-threonine 4-phosphate. Phosphohydroxythreonine arises from D-erythrose 4-phosphate, a product of decomposition of D-fructose 6-phosphate. Non-phosphorylated forms (pyridoxal, pyridoxol and pyridoxamine) are produced by hydrolysis of the corresponding phosphates. Animals do not synthesise vitamin Bg de novo, only convert the non-phosphorylated forms in the Hver, erythrocytes, and other tissues into the corresponding phosphates and the individual forms of each other. Pyridoxal 5 -phosphate arises by oxidation of pyridoxol 5Lphosphate and transamination of pyridoxal 5 -phosphate provides pyridoxamine 5Lphosphate. Both these forms of vitamin Bg are catalytically active. Pyridoxal 5 -phosphate... 

M. Gutierrez, T. Patella, J. Joglar, J. Bujons, P. Qapes, Strucmie-guided redesign of D-fructose-6-phosphate aldolase from E. coli remarkable activity and selectivity towards acceptor substrates by two-point mutation, Chem. Commun. 47 (2011) 5762-5764. 

Szekrenyi, A., Soler, A., Garrabou, X., Guerard-Helaine, C., Parella, T, Joglar, J., Lemaire, M., Bujons, J., and Clapes, R, Engineering the donor selectivity of D-fructose-6-phosphate aldolase for biocatalytic asymmetric cross-aldol additions of glycolaldehyde. Chem. Eur. J. 2014, 20 (39), 12572-12583. 

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