Nucleolus granular component

Close observations of immunofluorescence signals showed that there are three different staining patterns, which correspond to three different nucleolar compartments FC (Fibrillar center), DFC (dense fibrillar component), and GC (granular component). Nucleolus is surrounded by heterochromatin. When the cells are in very active state of its proliferation, the nucleolar compartments and heterochromatin are integrated into a highly intricate structure called nucleolonema . A recent study has suggested that the chromatin associated with the nucleolus is less mobile than... 

Fig.l Electron micrograph of an interphase nucleolus illustrating the component parts of fibrillar center (FC), dense fibrillar component (DFC), and granular component (GC). Scale bar, 0.5 /um. 

Fig. 6 Distribution of flbrillar centers in PHA-stimulated human lymphocytes at 0 (a and d), 24 (b and e), and 48 hr (c and f) as visualized by conventional electron microscopy (a-c) and by indirect immunofluorescence (d-f) using a human autoantibody to RNA polymerase I. FC, Fibrillar center, DFC, dense flbrillar component G, granular component. The resting, unstimulated lymphocyte (a and d) contains a single large FC (arrowhead in d) that increases in number and decreases in size as the nucleolus grows in size with continued stimulation, (a-c) Scale bar, 0.5 /zm. (d-f) Scale bar, 5 /i.m.

Anti-Pm-Scl 75- and 100-kDa proteins of the Pm-Scl complex Granular component of nucleolus... 

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