Nucleic acids relative fluorescence intensities

Table I. Relative Fluorescence Intensities Obtained from Various Types of Nucleic Acids...

One of the limitations associated with the use of fluorogenic dyes as labels for nucleic acids is the relatively high background fluorescence intensity in solution. This problem can be ameliorated by the use of asymmetric cyanine dyes. Another approach that can achieve low fluorescence background is based on the use of FRET pairs. If the acceptor and donor are in close proximity to... 

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