Nucleic acids membrane filtration

Figure 11.9 Production of rhGH in . coli (as an intracellular protein). Subsequent to fermentation, the cells are collected by centrifugation or filtration. After homogenization, nucleic acids and some membrane constituents are precipitated by the addition of polyethyleneimine. Ammonium sulfate precipitation of the supernatant concentrates the crude rhGH preparation. Chromatographic purification follows, as illustrated...

All biochemical laboratory activities, whether in education, research, or industry, are replete with techniques that must be carried out almost on a daily basis. This chapter outlines the theoretical and practical aspects of some of these general and routine procedures, including use of buffers, pH and other electrodes, dialysis, membrane filtration, lyophilization, centrifugal concentration, and quantitative methods for protein and nucleic acid measurement. 

Membrane chromatography systems include microporous or macroporous membranes that contain functional ligands attached to their inner pore structure, which act as adsorbents. In this sense, membrane chromatography is a hybrid combination of liquid chromatography and membrane filtration. Its most important potential applications include separations of biomolecules, such as proteins, polypeptides, and nucleic acids (85,86). 

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