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Nucleic acid cleavage endonucleases

This zinc-dependent endonuclease [EC 3.1.30.1] can act on either ribo- or deoxyribonucleic acids, with a preference for single-stranded substrates. It catalyzes the endonucleolytic cleavage of those nucleic acids to 5 -phospho-mononucleotide and 5 -phosphooligonucleotide end-products. [Pg.515]

Cleavage of deoxyoxanosine-containing oligodeoxyribonucleotides by bacterial endonuclease V. Nucleic Acids Res., 32, 4071-4080. [Pg.45]

Nucleases a group of hydrolytic enzymes which cleave nucleic acids. Exonucleases attack the nucleic acid molecule at its terminus, whereas endonucleases are able to catalyse a hydrolytic cleavage within the polynucleotide chain. Deoxyribonucleases (DNAases) are specific for DNA, ribonucleases (RNAases) for RNA. All N. are Phosphodiesterases (see) they catalyse the hydrolysis of either the 3 or 5 bond of the 3, 5 -phosphodiester linkage. Ribonuclease (see) has been extensively studied. [Pg.450]

Fig. 1 Nucleic acid samples available for electrochemical experiments, (a, b) naturally occurring DNAs (bl) dsDNA fragments of defined lengths and nucleotide sequences can be conveniently prepared by cleavage with restriction endonucleases (c) NAs (both DNA and RNA) synthesized by enzymes (d) fully synthetic DNAs and RNAs of limited lengths (e) PCR, can amplify the desired DNA segment from template DNA. ds, double-stranded ss, single-stranded, kbp, kilobase pairs. Covalently closed circles of sc, supercoiled and rel, relaxed DNA. oc, open circular DNA (containing at least one interruption of the sugar-phosphate backbone) lin, linear DNA. See text for more details. Fig. 1 Nucleic acid samples available for electrochemical experiments, (a, b) naturally occurring DNAs (bl) dsDNA fragments of defined lengths and nucleotide sequences can be conveniently prepared by cleavage with restriction endonucleases (c) NAs (both DNA and RNA) synthesized by enzymes (d) fully synthetic DNAs and RNAs of limited lengths (e) PCR, can amplify the desired DNA segment from template DNA. ds, double-stranded ss, single-stranded, kbp, kilobase pairs. Covalently closed circles of sc, supercoiled and rel, relaxed DNA. oc, open circular DNA (containing at least one interruption of the sugar-phosphate backbone) lin, linear DNA. See text for more details.
Noskov VN, Segall-Shapiro TH, Chuang RY (2010) Tandem repeat coupled with endonuclease cleavage (TREC) a seamless modification tool for genome engineering in yeast. Nucleic Acids Res 38 2570-2576... [Pg.180]

See other pages where Nucleic acid cleavage endonucleases is mentioned: [Pg.223]    [Pg.353]    [Pg.357]    [Pg.452]    [Pg.356]    [Pg.368]    [Pg.466]    [Pg.250]    [Pg.1673]    [Pg.92]    [Pg.314]    [Pg.312]    [Pg.244]    [Pg.250]    [Pg.147]    [Pg.465]    [Pg.1171]    [Pg.760]    [Pg.469]    [Pg.739]    [Pg.365]    [Pg.288]    [Pg.451]    [Pg.270]    [Pg.1202]    [Pg.109]    [Pg.149]   
See also in sourсe #XX -- [ Pg.273 , Pg.274 , Pg.275 , Pg.276 , Pg.277 , Pg.278 , Pg.279 ]



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Acidic cleavage

Cleavage acids

Cleavage endonucleases

Endonucleases

Nucleic acids cleavage

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