Nuclear transplantation, transgenic

Amount of hormone Prime two fanales the day before the nuclear transplanations with 15U PMSG. Inject the females 3 h before the nuclear transplantation with 150U HCG and leave frogs at 22-24 C. We use two females for two rounds of the nuclear transplantation a day. If more rounds of transgenesis are needed in the same day, inject another two females 1 or 2h after the first pair. This will allow for four rounds of transgenics, each around Ih apart. 

Kroll KL, Amaya E (1996) Transgenic Xenopus anbryos fiom sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development... 

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